Document Text Contents
Page 10
10 | P a g e
MATERIALS AND APPARATUS
MATERIALS: carnoy fixative, holding solution(70% ethanol), HCl acid( 18%), toluidine
blue.
APPARATUS : microscope slides, coverslips, small beakers, watch glasses, blades,
Forceps, filter paper, compound microscope.
METHOD
Preparing the sample
1. Sample of onion root cells is obtained from the holding solution.
2. Two cups of solutions are prepared. The first cup contains HCl acid while the
second one contains carnoy solution.
3. By using a forcep, an onion root tip is transferred into HCl acid solution for four
minutes.
4. Then, the root tip is transferred into carnoy solution for four minutes.
5. By using a blade, 1 mm of the root tip is cut and is put on microscope slide.
6. A few drops of toluidine blue is dropped into the root tip and is left for two
minutes. Then, a few drops of water is put onto the root tip to dilute the
concentrated toluidine blue.
7. By using a filter paper, the stain surround the root tip is blotted away.
8. Then, a cover slip is put over the specimen.
9. The slide is covered with a paper tissue and the cover slip is pressed with the
thumb.
10. Then, the slide is observed under microscope.
Page 18
18 | P a g e
REFERENCES AND BIBLIOGRAPHY
FROM INTER NET
2011. Available from: http://www.practicalbiology.org/areas/advanced/cells-to-
systems/cell-division/investigating-mitosis-in-allium-root-tip-
squash,121,EXP.html. Accessed on 1
st
February 2011
2011. Available from : http://en.wikipedia.org/wiki/Mitosis. Accessed on 1st
February 2011
2011. Available from : http://www.123helpme.com/preview.asp?id=156102.
Accessed on 1
st
February 2011
2011. Available from : http://www.experiment-resources.com/validity-and-
reliability.html. Accessed on 1st February 2011
2011. Available from: http://staff.jccc.net/pdecell/celldivision/oniontip.html.
Accessed on 1st February 2011
FROM BOOK
Angela,H. et al . 2008. Voice of Genome. In Salters-Nuffield Advanced Biology
or Edexcel AS Biolog, p.116-117. London. Edexcel Pearson.
10 | P a g e
MATERIALS AND APPARATUS
MATERIALS: carnoy fixative, holding solution(70% ethanol), HCl acid( 18%), toluidine
blue.
APPARATUS : microscope slides, coverslips, small beakers, watch glasses, blades,
Forceps, filter paper, compound microscope.
METHOD
Preparing the sample
1. Sample of onion root cells is obtained from the holding solution.
2. Two cups of solutions are prepared. The first cup contains HCl acid while the
second one contains carnoy solution.
3. By using a forcep, an onion root tip is transferred into HCl acid solution for four
minutes.
4. Then, the root tip is transferred into carnoy solution for four minutes.
5. By using a blade, 1 mm of the root tip is cut and is put on microscope slide.
6. A few drops of toluidine blue is dropped into the root tip and is left for two
minutes. Then, a few drops of water is put onto the root tip to dilute the
concentrated toluidine blue.
7. By using a filter paper, the stain surround the root tip is blotted away.
8. Then, a cover slip is put over the specimen.
9. The slide is covered with a paper tissue and the cover slip is pressed with the
thumb.
10. Then, the slide is observed under microscope.
Page 18
18 | P a g e
REFERENCES AND BIBLIOGRAPHY
FROM INTER NET
2011. Available from: http://www.practicalbiology.org/areas/advanced/cells-to-
systems/cell-division/investigating-mitosis-in-allium-root-tip-
squash,121,EXP.html. Accessed on 1
st
February 2011
2011. Available from : http://en.wikipedia.org/wiki/Mitosis. Accessed on 1st
February 2011
2011. Available from : http://www.123helpme.com/preview.asp?id=156102.
Accessed on 1
st
February 2011
2011. Available from : http://www.experiment-resources.com/validity-and-
reliability.html. Accessed on 1st February 2011
2011. Available from: http://staff.jccc.net/pdecell/celldivision/oniontip.html.
Accessed on 1st February 2011
FROM BOOK
Angela,H. et al . 2008. Voice of Genome. In Salters-Nuffield Advanced Biology
or Edexcel AS Biolog, p.116-117. London. Edexcel Pearson.
