Download mitosis report PDF

Titlemitosis report
TagsMitosis Cell Cycle Biology Earth & Life Sciences
File Size597.8 KB
Total Pages18
Document Text Contents
Page 10

10 | P a g e

MATERIALS AND APPARATUS

MATERIALS: carnoy fixative, holding solution(70% ethanol), HCl acid( 18%), toluidine

blue.

APPARATUS : microscope slides, coverslips, small beakers, watch glasses, blades,

Forceps, filter paper, compound microscope.

METHOD

Preparing the sample

1. Sample of onion root cells is obtained from the holding solution.

2. Two cups of solutions are prepared. The first cup contains HCl acid while the

second one contains carnoy solution.

3. By using a forcep, an onion root tip is transferred into HCl acid solution for four 

minutes.

4. Then, the root tip is transferred into carnoy solution for four minutes.

5. By using a blade, 1 mm of the root tip is cut and is put on microscope slide.

6. A few drops of toluidine blue is dropped into the root tip and is left for two

minutes. Then, a few drops of water is put onto the root tip to dilute the

concentrated toluidine blue.

7. By using a filter paper, the stain surround the root tip is blotted away.

8. Then, a cover slip is put over the specimen.

9. The slide is covered with a paper tissue and the cover slip is pressed with the

thumb.

10. Then, the slide is observed under microscope.

Page 18

18 | P a g e

REFERENCES AND BIBLIOGRAPHY

 FROM INTER NET
 2011. Available from: http://www.practicalbiology.org/areas/advanced/cells-to-

systems/cell-division/investigating-mitosis-in-allium-root-tip-
squash,121,EXP.html. Accessed on 1

st
February 2011

 2011. Available from : http://en.wikipedia.org/wiki/Mitosis. Accessed on 1st

February 2011

 2011. Available from : http://www.123helpme.com/preview.asp?id=156102.

Accessed on 1
st

February 2011

 2011. Available from : http://www.experiment-resources.com/validity-and-

reliability.html. Accessed on 1st February 2011

 2011. Available from: http://staff.jccc.net/pdecell/celldivision/oniontip.html.
Accessed on 1st February 2011

 FROM BOOK 
 Angela,H. et al  . 2008. Voice of Genome. In Salters-Nuffield Advanced Biology

or Edexcel AS Biolog, p.116-117. London. Edexcel Pearson.

Similer Documents